Role of Peritoneal Macrophages in Cytomegalovirus-induced Acceleration of Autoimmune Diabetes in BB-rats

BACKGROUND As one of the natural perturbants, infection with cytomegalovirus (CMV) is believed to play a role in the development of Type I diabetes. Using the DP-BB rat model for autoimmune diabetes, we here report about possible mechanisms responsible for R(at)CMV-induced accelerated onset of diabetes. METHODS Rats were i.p. infected with 2 x 10(6) plaque forming units (pfu) RCMV and followed for diabetes development. Presence of RCMV antigens and DNA was analyzed by immunohistochemistry and PCR on pancreatic tissue and isolated islets. The effect of viral infection on peritoneal macrophages (pMphi) and diabetes development was studied by analyzing numbers of pMphi, virus permissiveness and by depletion of this subset by peritoneal lavage. RESULTS RCMV accelerated onset of diabetes without infecting pancreatic islets. Immunohistochemistry and PCR on pancreas and isolated islets indicated that islets are non-permissive for RCMV. Infection results in an influx of pMphi 1 day p.i. of which approximately 0.05% showed signs of reproductive infection. Depletion of pMphi on days 1-3 p.i. completely counteracted the accelerating effect of RCMV. INTERPRETATION RCMV accelerates onset of diabetes without infecting pancreatic islets. pMphi might function as an carriage to disseminate virus to the pancreas where they enhance activation of autoreactive T cells resulting in accelerated onset of diabetes.